Feasibility of the use of capillary electrophoresis for the study of vldl assembly intermediates
Abstract
The chicken has long been a model used for the study of plasma lipoproteins due
to the ability to increase VLDL production by administration of estrogen. In this study
we were able to demonstrate successful isolation of VLDL assembly intermediates from
the livers of hens, roosters, and estrogen treated rosters. Particle diameter of first step
particles, as determined by dynamic laser light scattering, was decreased from an
average diameter of 31.5 nm in untreated birds, to 16.1 nm 12 hours after estrogen
treatment. Effects of estrogen waned after 24 hours and particle diameter of first step
particles increased to an average of 23.9 nm. These assembly intermediates, as well as
plasma VLDL and VLDLy, were successfully studied using capillary electrophoresis
(CE). Effective mobilities of intact plasma VLDL and first step particles decreased after
estrogen administration. Hen VLDL showed a single uniform peak whereas rooster
VLDL separated into distinct ÂsubclassesÂ. Delipidated VLDL, VLDLy and first step
assembly intermediates were also successfully separated using CE. This thesis is dedicated to my family who always encouraged me through this process.
Citation
White, Elizabeth Anne (2005). Feasibility of the use of capillary electrophoresis for the study of vldl assembly intermediates. Master's thesis, Texas A&M University. Texas A&M University. Available electronically from https : / /hdl .handle .net /1969 .1 /3777.