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dc.contributor.advisorKladde, Michael
dc.creatorJessen, Walter Joseph
dc.date.accessioned2006-04-12T16:06:17Z
dc.date.available2006-04-12T16:06:17Z
dc.date.created2004-12
dc.date.issued2006-04-12
dc.identifier.urihttp://hdl.handle.net/1969.1/3306
dc.description.abstractIn eukaryotes, the organization of DNA into chromatin is a primary determinant of gene expression. Positioned nucleosomes in promoter regions are frequently found to regulate gene expression by obstructing the accessibility of cis-regulatory elements in DNA to trans-factors. This dissertation focuses on the chromatin structure and remodeling program at the S. cerevisiae PHO5 promoter, extending the use of DNA methyltransferases as in vivo probes of chromatin structure. Our studies address the diversity of histone-DNA interactions in vivo by examining nucleosome conformational stabilities at the PHO5 promoter. We present high-resolution chromatin structural mapping of the promoter, required to relate in vivo site accessibility to nucleosome stability and show that the PHO5 promoter nucleosomes have different accessibilities. We show a correlation between DNA curvature and nucleosome positioning, which is consistent with the observed differences in accessibility/stability. Kinetic analyses of the chromatin remodeling program at PHO5 show that nucleosomes proximal to the enhancer are disrupted preferentially and prior to those more distal, demonstrating bidirectional and finite propagation of chromatin remodeling from bound activators and providing a novel mechanism by which transactivation at a distance occurs.en
dc.format.extent2738887 bytesen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherTexas A&M University
dc.subjectPHO5en
dc.subjectpromoteren
dc.subjectchromatinen
dc.subjectremodelingen
dc.subjectnucleosomeen
dc.subjectaccessibilityen
dc.titleChromatin dynamics at the Saccharomyces cerevisiae PHO5 promoteren
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentBiochemistry and Biophysicsen
thesis.degree.disciplineBiochemistryen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.levelDoctoralen
dc.contributor.committeeMemberHu, James
dc.contributor.committeeMemberPolymenis, Michael
dc.contributor.committeeMemberThomas, Terry
dc.type.genreElectronic Dissertationen
dc.type.materialtexten
dc.format.digitalOriginborn digitalen


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