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dc.contributor.advisorKladde, Michael P.
dc.creatorDhasarathy, Archana
dc.date.accessioned2006-04-12T16:05:34Z
dc.date.available2006-04-12T16:05:34Z
dc.date.created2004-12
dc.date.issued2006-04-12
dc.identifier.urihttp://hdl.handle.net/1969.1/3272
dc.description.abstractIn higher eukaryotes, DNA is packaged with histones and other proteins into chromatin. While this is important in the control of unwanted gene expression, chromatin also serves as a barrier to many vital functions in the cell. Therefore, cells have evolved many different types of chromatin remodeling enzymes to contend with this inhibitory structure and enable gene expression and other functions. The Saccharomyces cerevisiae PHO5 gene is triggered in response to phosphate starvation. In this study, I evaluate the chromatin remodeling requirements of this gene with respect to the multisubunit complexes SWI/SNF and SAGA. I show, for the first time, physical recruitment of SWI/SNF to the PHO5 promoter. I also demonstrate the role of promoter occupancy in influencing requirements for chromatin remodeling enzymes. Further, I describe various interactions between these two complexes at the PHO5 promoter. This study presents evidence for the first instance of excess recruitment of an ATP-dependent remodeler potentially compensating for the lack of a histone acetyltransferase.en
dc.format.extent10159054 bytesen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherTexas A&M University
dc.subjectchromatinen
dc.subjectGcn5en
dc.subjectPHO5en
dc.subjectgene expressionen
dc.subjectSAGAen
dc.subjectSWI/SNFen
dc.titleInterplay between promoter occupancy and chromatin remodeling requirements in transactivation of the S.cerevisiae PHO5 geneen
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentBiochemistry and Biophysicsen
thesis.degree.disciplineGeneticsen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.levelDoctoralen
dc.contributor.committeeMemberPeterson, David O.
dc.contributor.committeeMemberShippen, Dorothy E.
dc.contributor.committeeMemberJiang, Yi Wei
dc.type.genreElectronic Dissertationen
dc.type.materialtexten
dc.format.digitalOriginborn digitalen


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