| dc.description.abstract | This project contributes to the development of a biocompatible, multi-analyte biotransducer and associated instrumentation that serves as a minimally invasive implantable biosensor system to improve patient stratification, guide resuscitation and monitor the stabilization of hemorrhaging trauma patients. The pH-responsive element of the multi-analyte biotransducer, with sensing elements for glucose, lactate, pH, potassium and partial pressure of oxygen (pO2), was addressed. A pH-responsive hydrogel for the measurement of acidosis under physiological conditions was synthesized from poly(2-hydroxyethyl methacrylate-co-poly(ethylene glycol) methacrylate)-based hydrogels that were molecularly engineered with AEMA and DMAEMA and surveyed for the distribution of water states within the hydrogel. It was found that bound water, not total hydration, correlated strongly with the biotechnical properties, determined the membrane resistance and thereby the pH sensitivity of hydrogels. Surface modification of electrodes was accomplished using polypyrrole and bioactive hydrogels to reduce the charge transfer resistance for ABIO-BIO interface engineering.
Using multiplexed biomarker inputs and physician expert scoring, a Hemorrhage Intensive Severity and Survivability (HISS) score was obtained from a fusion of input data and could correlate to survivability using data-driven prediction models. For real-time monitoring, this biotransducer was interfaced externally with hardware components including front-end electronics and a display readout. This microsystem will be used as an intramuscular indicator for the pathophysiology of hemorrhage | |
