The Long-Term Effects of Unloading on the Transdifferentiation of Condylar Chondrocytes
dc.contributor.advisor | Jing, Yan | |
dc.contributor.advisor | Buschang, Peter | |
dc.creator | Janssen, Abbey Elizabeth | |
dc.date.accessioned | 2023-02-07T16:16:03Z | |
dc.date.available | 2024-05-01T06:06:08Z | |
dc.date.created | 2022-05 | |
dc.date.issued | 2022-04-13 | |
dc.date.submitted | May 2022 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/197288 | |
dc.description.abstract | Background The role of chondrocyte transdifferentiation into bone cells during postnatal condylar growth remains unknown. Purpose: To quantify the long-term effects of masticatory forces on condylar chondrocyte transdifferentiation. Research Design: Chondrocyte tracing was performed in 12 Aggrecan-CreERT2;R26RTdTomato;2.3Col1a1-GFP and 12 Col10a1-Cre; R26RTdTomato;2.3Col1a1-GFP mice. After Tamoxifen injections at 3 weeks, they were randomly divided into soft and hard food diet groups and followed for 6 weeks. 2D and 3D µCT, H&E staining, cell proliferation, immunostaining for chondrogenic markers, and cell lineage tracing analyses were performed. Results: Mice on soft diet showed significantly (p<.05) smaller mandibular length, less chondrocyte proliferation and maturation, and fewer chondrocyte-derived bone cells in the condylar head (30% less) and mid-condylar process (28% less). Conclusions: Masticatory function affects condylar chondrogenesis and chondrocyte transdifferentiation, which may partially explain differences in postnatal condylar growth. | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | |
dc.subject | chondrocytes | |
dc.subject | diet modification | |
dc.subject | condyle | |
dc.subject | transdifferentiation | |
dc.title | The Long-Term Effects of Unloading on the Transdifferentiation of Condylar Chondrocytes | |
dc.type | Thesis | |
thesis.degree.department | Orthodontics | |
thesis.degree.discipline | Oral Biology | |
thesis.degree.grantor | Texas A&M University | |
thesis.degree.name | Master of Science | |
thesis.degree.level | Masters | |
dc.contributor.committeeMember | Feng, Jian Q. | |
dc.contributor.committeeMember | Tadlock, Larry | |
dc.type.material | text | |
dc.date.updated | 2023-02-07T16:16:04Z | |
local.embargo.terms | 2024-05-01 | |
local.etdauthor.orcid | 0000-0003-0690-9656 |
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