| dc.description.abstract | The antibacterial activity against pathogenic bacteria and stimulatory effect of barberry polyphenols nanoparticle (NP) with Pluronic® F127 copolymer on the survival and growth rate of probiotic bacteria through an in vitro gastrointestinal tract (GIT) and colonic fermentation models were investigated. Antibacterial activity of free polyphenolic extract showed greater antibacterial activity against Gram-positive bacteria of Listeria monocytogenes, Enterococcus faecium, and Staphylococcus aureus compared to that of Gram-negative bacteria of Escherichia coli O157:H7,
Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa ranging from 0.3125 mg/mL to 10 mg/mL. The antibacterial activity of the treatments was dose-dependent. The susceptibility of Gram-positive microorganisms was the order of S. aureus > E. faecium > L. monocytogenes and Gram-negative bacteria in order of P. aeruginosa > S. Typhimurium > E. coli O157:H7.
The stability of barberry extracts was also evaluated in a simulated gastrointestinal digestion model that mimicked oral, gastric, small intestinal, and colon conditions through manipulation of digestive enzymes and pH values at 6.8, 2.5, 5.8, and 6.8, respectively at 3 min, 2 h, and 2 h at 37°C. The effects of the free treatments on the growth of probiotic bacteria including Lactobacillus spp, Bifidobacterium spp., co-culture, and E. coli O9:H4 strain HS were then investigated at concentrations ranging of 240 mg/L, 480 mg/L, 720 m/L, 960 mg/L, and 1200 mg/L at 37°C for 48 h. The concentration of 240 mg/L was found to optimally promote bacterial growth by 88% when co-culture bacteria were treated with free extract, whereas the growth values were up to 82% for Lactobacillus spp. and Bifidobacterium spp. However, the concentration of 720 mg/L was the most effective concentration among other concentrations that stimulated the growth of E. coli HS by 43%. | |
