Anticancer Effect of Physically Fractionated Bioactive Compounds of Green Coffee Bean in Ovarian Cancer Cell

Abstract

Ovarian cancer is one of the leading causes of female cancer death worldwide. Current treatment of the disease is by chemically-produced synthetic drugs; However, chemical drugs mostly have toxicity that are harmful for substantial treatment. Physically-extracted green coffee bean (GCB) extract may have anticancer effect to ovarian cancer cells by the substances of caffeine, chlorogenic acids, and specific proteins. In order to validate dose dependent cell killing efficacy of the physically extracted GCB, 3*10^4 cells of ovarian cancer cell line (A2780) were seeded in a 96 well plate and were treated with the GCB extract in various concentrations from 0.1 μg/mL to 3000 μg/mL for 24 hours. The extract may be used in the development of novel anticancer drugs in future. Distinct and physical extraction method of our research allows higher yield of significant substances than most of chemical extraction method. Our method is also environmentally friendly since the process does not involve chemicals.