Genotyping and Mitochondrial Phylogenomics of the Asexual Fish Poeciliopsis Monacha-Latidens
Abstract
The freshwater livebearing fish genus Poeciliopsis has served as a study system for the evolution of sex, due to the presence of asexually reproducing (gynogenetic and hybridogenetic) biotypes that originated through hybridization. Previous studies inferred the evolutionary origin of most Poeciliopsis asexual biotypes, but not of the diploid hybridogen (=hemiclone) P. monacha-latidens. We used phylogenetic analyses of whole mitochondrial genomes to infer that P. monacha-latidens had multiple independent origins. Within the Rio Mocorito (its southernmost limit), the monophyly of P. monacha-latidens and P. monacha-lucida and their sharing of identical mitochondrial haplotypes, suggests that they represent the same hemiclonal lineage, utilizing two sperm donor species. We provide the first report of heteroplasmy in Poeciliopsis, and of existence of P. viriosa x P. latidens hybrids. In addition, as an alternative to allozyme-based genotyping for Poeciliopsis, we optimized a DNA-based protocol to diagnose the presence or absence of a nuclear P. latidens allele.
Citation
Wang, Yunyang (2021). Genotyping and Mitochondrial Phylogenomics of the Asexual Fish Poeciliopsis Monacha-Latidens. Master's thesis, Texas A&M University. Available electronically from https : / /hdl .handle .net /1969 .1 /196037.