Transcriptome Assembly and Characterization of the Benthic Annelid Paramphinome jeffreysii
Abstract
Species of the phylum Annelida have been essential as model organisms in the studies of biology, neurobiology, evolution, ecology, and phylogenomics. Prior work using genomics and transcriptomics has provided new insights into the evolution of Annelida, such as phylogenetic relationships, life history, and lifestyle adaptations. Little biological information is known about the amphinomid Paramphinome jeffreysii. Although transcriptome data have been available since 2014, complete annotations of gene content is not publically available. The objective of this research is to annotate the transcriptome of P. jeffreysii in order to contribute towards a more comprehensive understanding of the biological pathways, cellular components, and molecular functions of the species. Cellular and metabolic processes, as well as biological regulation were among the top biological processes discovered, while binding, catalytic activity, and transporter activity were among the top molecular functions found. The top-hit species included a brachiopod, Lingula anatina, as well as Capitella teleta (second top hit), and several mollusks, highlighting the lack of available comparable annotated data for Amphinomida, and Annelida in general within public databases. Therefore, the continued exploration of transcriptomics in non-model organisms, such as P. jeffreysii, allows for continuing comparative research.
Citation
Martinez, Irene (2019). Transcriptome Assembly and Characterization of the Benthic Annelid Paramphinome jeffreysii. Undergraduate Research Scholars Program. Available electronically from https : / /hdl .handle .net /1969 .1 /194469.