Phosphonate Functionalized Inorganic-Organic Hybrid Hydrogels for Bone Regeneration
Abstract
Studies have indicated that the physical and chemical properties of scaffolds alone, without exogenous growth factors, can guide mesenchymal stem cell differentiation toward bone tissue regeneration. Ceramics, containing inorganics and phosphonates, are primarily used to impart osteoinductivity and bioactivity. However, these materials are brittle in nature which may lead to post-surgical fracture. Our lab has previously demonstrated osteoinductivity and bioactivity in a non-brittle, interconnected macroporous system with the presence of methacrylated star poly(dimethylsiloxane) (PDMSstar-MA) within a normally biologically inert poly(ethylene glycol) (PEG-DA) hydrogel. Because phosphorus-containing materials are known to increase osteoblastic differentiation and facilitate mineralization, this study aims to enhance this scaffold through functionalization of the inorganic PDMSstar-MA component with a phosphonate group. This was accomplished through the development and inclusion of a novel polymer, phosphonate-containing PPMS-DA. Key material properties were evaluated (i.e. modulus, swelling, degradation and bioactivity) and compared to a PEG-DA hydrogel and the previously studied hybrid hydrogel, PDMS:PEG. The phosphonate results indicated a more uniform distribution, similar mechanical properties and bioactivity, and increased degradation rate useful to promote tissue infiltration.
Subject
BioactiveBone
Exogenous growth factors
Hybrid
Hydrogels
Hydroxyapatite
Inorganic
Interconnected macropores
Material-guided approach
Mineralization
Osteoblastic differentiation
Osteoconductive
Osteoinductive
PDMSstar-MA
PEG-DA
Pendant phosphonate group
Phosphonate
Polymer synthesis
PPMS-DA
Regeneration
Salt templating
Scaffold
Solvent induced phase separation
Tissue engineering
Citation
Jones, Sarah Katlyn (2020). Phosphonate Functionalized Inorganic-Organic Hybrid Hydrogels for Bone Regeneration. Undergraduate Research Scholars Program. Available electronically from https : / /hdl .handle .net /1969 .1 /194457.