| dc.description.abstract | Antibiotic resistance emerged shortly after the introduction of antibiotics into the field of medicine, bringing about a challenging concern. Resistance to antibiotics is encoded by antibiotic resistance genes, among other mechanisms. Antibiotic resistance genes are highly regulated genes that are expressed when antibiotics are introduced. The current research focuses on one resistance gene ytbD, encoded by Bacillus subtilis. This research will give an insight into ytbD regulation by observing the spatial and temporal expression of a luciferase reporter gene fused to the ytbD promoters. The expression pattern is observed under the control of different lengths of ytbD promoters when ribosome-targeting, including chloramphenicol, and nonribosom-targeting antibiotics are introduced. To build the different lengths of the promoters, we designed primers that will include or exclude predicted regulatory sequences during the engineering of the reporter strains. In doing so, we are trying to test if these upstream sequences play a role in the regulation of ytbD and whether antibiotics will affect the regulation pattern. | en |
