dc.creator | Chen, Zhilei | |
dc.creator | Ramirez, Miguel A. | |
dc.creator | Guan, Dongli | |
dc.date.accessioned | 2019-06-17T17:15:07Z | |
dc.date.available | 2019-06-17T17:15:07Z | |
dc.date.issued | 2018-10-02 | |
dc.identifier.uri | https://hdl.handle.net/1969.1/177269 | |
dc.description.abstract | The present invention includes methods, compositions, uses, and kits for purifying a protein of interest (POI) comprising contacting a first fusion protein comprising the POI fused to the C-terminus of an intein C-fragment with a second fusion protein comprising an intein N-fragment and a purification tag to form a complex between the first fusion protein and the second fusion protein, cleaving the POI from the intein C-fragment, wherein the protein is released from the complex; and isolating the POI; the present invention also includes fusion proteins and vector. | en |
dc.language | eng | |
dc.publisher | United States. Patent and Trademark Office | |
dc.rights | Public Domain (No copyright - United States) | en |
dc.rights.uri | http://rightsstatements.org/vocab/NoC-US/1.0/ | |
dc.title | Intein mediated purification of protein | en |
dc.type | Utility patent | en |
dc.format.digitalOrigin | reformatted digital | en |
dc.description.country | US | |
dc.contributor.assignee | THE Texas A&M UNIVERSITY SYSTEM | |
dc.identifier.patentapplicationnumber | 14/760392 | |
dc.date.filed | 2014-01-10 | |
dc.publisher.digital | Texas A&M University. Libraries | |
dc.subject.cpcprimary | C07K 1/14 | |
dc.subject.cpcprimary | C07K 1/22 | |
dc.subject.cpcprimary | C07K 1/32 | |
dc.subject.cpcprimary | C12N 9/16 | |
dc.subject.cpcprimary | C07K 2319/20 | |
dc.subject.cpcprimary | C07K 2319/92 | |
dc.subject.cpcprimary | C12Y 301/00 | |