Solid phase fluorescence labeling reagents and uses thereof
Abstract
A solid phase reagent and method for simultaneously capturing and fluorescently labeling an analyte with multiple reactive sites to provide a mono-labeled analyte are disclosed. The reagent can be used in the method and comprises a plurality of analyte-reactive groups tethered to a porous solid phase, wherein each analyte-reactive group is covalently attached to a fluorescent group either directly or indirectly, wherein the distance between adjacent analyte-reactive groups is greater than the gyration radius of a captured analyte; the fluorescent group is covalently attached to a cleavable anchor group either directly or through a first spacer; and the cleavable anchor group is covalently attached to the solid phase either directly or through a second spacer, wherein the solid phase is a porous solid or a porous gel.
Subject
C08F 20/26C07C 311/39
C07D 317/22
C09B 57/001
G01N 33/54306
G01N 33/54393
G01N 33/582
G01N 33/6809
C07C 2603/50
Y10T 436/173845
Collections
Citation
Vigh, Gyula; Estrada, Roy T. III (2018). Solid phase fluorescence labeling reagents and uses thereof. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /177233.