High-throughput mutagenized cell screening system for selective single cell extraction
Abstract
The subject invention pertains to a microfluidic apparatus and methods for screening and isolating a target cell from a population of cells. The apparatus comprises a first microfluidic layer comprising microfluidic channels; a second microfluidic layer comprising microfluidic channels; and a microfluidic cell analysis layer comprising a top hanging blocking structure located directly below each location where the first layer microfluidic channels overlap with the second layer microfluidic channels and a cell trap juxtaposed to each of the top hanging blocking structures. The top hanging blocking structures can close or open the juxtaposed cell trap when either or both the first or second layer microfluidic channels located directly above the top hanging blocking structure are sufficiently pressurized and/or sufficiently depressurized. The methods for screening and isolating a target cell from a population of cells comprise screening the population of cells using the apparatus and isolating the target cell interest therefrom.
Subject
C12Q 1/24B01L 3/502753
C12M 47/04
G01N 33/5005
B01L 2200/0668
B01L 2200/10
B01L 2300/0636
B01L 2300/0816
B01L 2300/0887
B01L 2300/123
B01L 2400/0655
B01L 2400/086
Collections
Citation
Han, Arum; Kim, Hyun Soo (2017). High-throughput mutagenized cell screening system for selective single cell extraction. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /177224.