Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells

Abstract

The present invention provides an alternative strategy for baculovirus-mediated foreign gene expression: the promoters from immediate-early or delayed-early baculovirus viral genes were used to obtain the continuous expression of foreign genes in stably-transformed insect cells. The IE1 or the 30K promoters from Autographa californica nuclear polyhedrosis virus can drive the continuous expression of a foreign gene in insect cells. IE1-β-galactosidase or 39K-β-galactosidase constructs were used in combination with the IE1-neomycin resistan The Government may have rights in this invention pursuant to a funding agreement with the National Science Foundation (NSF), Grant No. DMB-88 04732.