Use of bacterial beta-lactamase for in vitro diagnostics and in vivo imaging, diagnostics and therapeutics
Abstract
Provided herein are assays and in vitro methods to determine susceptibility to a drug effective against a pathogenic bacteria, for example, a pathogenic Mycobacteria, that has a beta-lactamase activity. An excitation wavelength is delivered to a biological sample obtained from a subject having an infection from the pathogenic bacteria in the presence of a beta-lactamase substrate. The intensity of a signal, such as a fluorescent, luminescent or colorimetric signal, at an emission wavelength of a product of the beta-lactamase on the subject is correlated to drug susceptibility. Also provided is an assay system for monitoring drug susceptibility of a pathogenic bacteria comprising color-producing substrates for a beta-lactamase of the pathogenic bacteria, an assay device for visibly detecting a product of the beta-lactamase on the substrate thereof and a reader configured to quantify the visibly detected product.
Subject
C12Q 1/34A61K 49/0013
A61K 49/0021
A61K 49/0028
A61K 49/0032
A61K 49/0041
A61K 49/0052
C09B 57/02
C12Q 1/04
C12Q 1/66
G01N 2333/986
Collections
Citation
Cirillo, Jeffrey D.; Rao, Jianghong (2016). Use of bacterial beta-lactamase for in vitro diagnostics and in vivo imaging, diagnostics and therapeutics. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /177169.