Isolation of proteins involved in posttranscriptional gene silencing and methods of use
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The present disclosure includes methods of using viral and plant proteins to regulate silencing in plants such as monocots. Some embodiments include methods of increasing expression of transgenes in plants. Such methods may include selecting a protein involved in post-translational gene silencing (PTGS), for e.g., a protein active in PTGS or a suppressor of PTGS, in a plant and transforming the plant with a nucleic acid encoding the protein involved in PTGS wherein expression of the protein involved in PTGS increases expression of the transgene in the plant. One non-limiting example of such a protein is the Sorghum Mosaic Virus (SrMV) P1/HC-Pro. Methods of the disclosure may be used to regulate silencing of transgenes and to increase expression of transgenes in a variety of plants such as sugarcane, corn, sorghum, and/or rice.
Mirkov, Erik T.; Ingelbrecht, Ivan L. (2010). Isolation of proteins involved in posttranscriptional gene silencing and methods of use. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from