Method for producing a recombinant baculovirus expression vector
Abstract
A method for producing a recombinant baculovirus expression vector, capable of expression of a selected gene in a host insect cell, is disclosed. The preferred method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant shuttle vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant shuttle vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome.
Subject
435/69.51435/69.1
435/91.41
435/243
435/320.1
435/456
435/463
435/466
536/24.1
930/220
C12N 15/86
C07K 14/52
C07K 14/005
C07K 2319/09
C07K 2319/735
C07K 2319/00
C07K 2319/75
C12N 2710/14122
C12N 2710/14143
C07K 2319/02
Collections
Citation
Smith, Gale E.; Summers, Max D. (1988). Method for producing a recombinant baculovirus expression vector. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /176820.