Collagen-binding proteins from enterococcal bacteria
Abstract
A collagen-binding MSCRAMM entitled Ace from enterococcal bacteria is provided which was homologous to the ligand-binding region of Cna, the collagen-binding MSCRAMM from Staphylococcus aureus, and which can be utilized in a similar manner as other collagen-binding MSCRAMMs to inhibit adhesion of enterococcal bacteria to extracellular matrix proteins. The N-terminal region of Ace contained a region (residues 174-319), or A domain, which appears to be equivalent to the minimal ligand-binding region of the collagen-binding protein Cna (Cna 151-318), and contains several 47-residue tandem repeat units, called B domain repeat units, between the collagen-binding site and cell wall-associated regions. The Ace protein of the invention can thus be utilized in methods of preventing and/or treating enterococcal infection, and in addition, antibodies raised against Ace, or its A domain, can be used to effectively inhibit the adhesion of enterococcal cells to a collagen substrate. The Ace protein of the present invention is thus a functional collagen-binding MSCRAMM and can be utilized to treat or prevent invention in the same manner as other isolated MSCRAMMs have been utilized, namely in methods of treating or preventing infections and diseases caused by enterococcal bacteria.
Subject
536/23.7424/184.1
424/190.1
424/234.1
536/23.1
A61K 39/02
C07K 16/1271
C07K 16/1275
G01N 33/56938
A61K 2039/505
G01N 2333/315
Collections
Citation
Rich, Rebecca L.; Kriekemeyer, Bernd; Owens, Rick T.; Hook, Magnus; Murray, Barbara E.; Nallapareddy, Sreedhar R.; Weinstock, George M. (2005). Collagen-binding proteins from enterococcal bacteria. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /176784.