Developing Protein Tags for Secondary Ion Mass Spectrometry to Determine Protein Interaction
Abstract
The development of protein tags that enable the analysis of proteins with high multiplexity, in the context of cellular interactions. Nanoparticle and metal chelated tags were developed and utilized by Secondary Ion Mass Spectrometry (SIMS) for the detection and analysis of cell surface proteins. Small (5 nm in diameter) nanoparticles in addition to metal chelates were optimized for antibody conjugation and protein tagging. These tags feature unique metal signatures for easy detection and characterization using mass spectrometry. The tags were tested and characterized using massive cluster SIMS with event-by-event bombardment and detection and a time of flight (ToF) mass analyzer.
Subject
SIMSSecondary Ion Mass Spectrometry
protein tags
metal chelates
nanoparticles
massive cluster SIMS
event-by-event
time of flight
protein analysis
mass spectrometry
Citation
Kozman, Mena M (2019). Developing Protein Tags for Secondary Ion Mass Spectrometry to Determine Protein Interaction. Undergraduate Research Scholars Program. Available electronically from https : / /hdl .handle .net /1969 .1 /175399.