| dc.description.abstract | In this study, a hybrid of the bridge species Arachis vallsii Krapov. and W.C. Greg. (VSW 9902-1) and A. dardani Krapov. and W.C. Greg. (GK12946) was created to initiate an introgression pathway for movement of possible drought tolerance genes into the cultivated peanut (A. hypogaea L.). A hybrid between the two species was successfully created and confirmed based on leaf morphology, pollen counts and intermediated leaf morphology. One-hundred and seventy-five attempts were made to double the chromosome complement using 3 methods at concentrations of 0.02% and 0.03% colchicine for exposure times ranging from 6 to 24 hours. No attempt has been successful to date. In addition, a greenhouse transcriptome study with 7 day-imposed drought was conducted on A. dardani (12946) and the reference species A. ipaënsis (Krapov. and W.C. Greg.) (KGBPScS-30076) (B genome donor of the cultivated peanut). Differential gene expression analysis (EdgeR Test) of the normalized RPKM (Reads Per Kilobase Million mapped reads) values was conducted with a fold value ≥ abs (2) at the p ≤ 0.05 level using CLC Genomics Workbench v8. Significant transcript levels associated with drought tolerance were found in relation to the putative drought species (A. dardani (12946)), which have not been reported previously. Transcripts were identified that were higher between physiological states and between species. In total, 40 genes were identified for further study. | en |
