Detection and Identification of Live and Dead Bacteria by Spectroscopic Analysis and Designing a Novel Instrument for In-Situ Application
Abstract
Most of the illnesses in this world are caused by bacterial infections. Thus, their in situ detection and identification is a matter of worldwide concern in human pathology. This MS thesis research presents the in situ detection of the number of live and dead bacteria in an infected area, by means of absorption and fluorescence emission of the present analysis, as well as the flux of UV radiation needed to inactivate or kill 80-90% of the live bacteria. The effects of UV radiations upon various components of bacteria by spectroscopic analysis are discussed. With the help of this spectroscopic analysis, the design of hand-held synchronous spectrometer is proposed to detect and identify bacteria instantly and in situ. Fluorescence and absorption spectra of two different bacterial species (Escherichia coli, Rhodococcus opacus), two different bacteria proteins (tryptophan and tyrosine) and bacteria DNA have also been studied. The technique discussed in this research and proposed design of a novel hand-held instrument is expected to be able to perform live and dead bacteria identification in a much shorter period of time than the standard methods and in addition provide in situ detection of bacteria in wounds which is not possible with present methods. A nano-second time resolved spectroscopy system has been designed and implemented on an optical table and tested for proper operation, in order to do further research on DNA dimerization mechanism and singlet oxygen formation mechanism after illuminating methylene blue.
Citation
Khan, Kashfia Quader (2016). Detection and Identification of Live and Dead Bacteria by Spectroscopic Analysis and Designing a Novel Instrument for In-Situ Application. Master's thesis, Texas A & M University. Available electronically from https : / /hdl .handle .net /1969 .1 /174235.