Basic and Applied Studies of Cell-Matrix Interactions

Abstract

Extracellular matrix is a complex network of molecules that accomplishes various roles within each tissue. The mechanical properties and biochemical composition of the extracellular matrix vary considerably across tissues. In addition to providing structural support and elasticity, extracellular matrix properties influence various cellular behavior and processes like adhesion, differentiation and proliferation, among others. In these studies, I focused on elucidating the effects of changes in matrix composition and stresses on cellular architecture. In addition, the insights gained from these studies were utilized to develop a novel cell-based disease model of Malignant Bone Disease in which human mesenchymal stem cells and bone tumor cells are co-cultured on osteogenic microsphere in a rotating wall vessel to promote the growth of 3D tissue constructs.

Successful execution of these studies will lead to a range of new methods for the investigation of tumor-stem cell interactions without the limitations of 2D tissue culture or in vivo approaches. Furthermore, we expect that this approach will significantly accelerate drug research and substantially improve our understanding of tumor expansion and bone repair during long term exposure to micro-gravity.