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dc.creatorMetcalf, Megan
dc.date.accessioned2018-05-23T15:36:26Z
dc.date.available2018-05-23T15:36:26Z
dc.date.created2018-05
dc.date.submittedMay 2018
dc.identifier.urihttp://hdl.handle.net/1969.1/166521
dc.description.abstractSpecifically targeting cancer cells is much more efficient than non-targeted treatment because it decreases side effects and permits stronger treatment without as a risk of harming normal cells. Smart molecular probes and materials capable of passively or actively targeting cancerous cells show potential in cancer treatment for this reason. The combination of Metal-Organic Frameworks (MOFs) with actively targeting molecules provides an attractive way for targeted cancer theranostic methods. In this work, peptide sequences were synthesized that have high affinity and specificity towards proteins expressed on the surface of cancer cells. To introduce enough flexibility for targeted binding and provide extra functionality, the targeting peptide AP2H was further modified with polyethylene glycol (PEG) as a spacer molecule, and the peptide RGDG was used in place of RGD in order to use the additional glycine residue as a spacer molecule. After solid phase synthesis and cleavage, the resultant peptides were characterized with high-performance liquid chromatography (HPLC) and mass spectrometry (MS). Biocompatible metal-organic frameworks ZIF-8, UiO-66, and UiO-66-NH2 were synthesized. UiO-66-NH2 was functionalized with the peptide sequences to build a targetable delivery system using the MOF to carry molecules, such as fluorescent probes or anticancer drugs, directly to tumor cells by using the targeting peptides RGD and AP2H. Doxorubicin (DOX), a typical anticancer drug, was used as a model to determine whether the peptide-guided delivery system has the potential for carrying molecular cargos. UV-Vis spectrophotometry was used to quantitatively measure the uptake and subsequent release of DOX. The DOX-encapsulated MOF paired with a targeting peptide can be promising as a targetable delivery system for cancer treatment.en
dc.format.mimetypeapplication/pdf
dc.subjectMOFen
dc.subjectmetal organic frameworken
dc.subjecttargeting peptideen
dc.subjectcanceren
dc.subjecttheranosticsen
dc.subjectdrug deliveryen
dc.subjectencapsulationen
dc.subjectbioprobeen
dc.titleDesign, Synthesis and Characterization of Peptide-guided Nanomaterial for Cancer Targetingen
dc.typeThesisen
thesis.degree.departmentChemistryen
thesis.degree.disciplineChemistryen
thesis.degree.grantorUndergraduate Research Scholars Programen
thesis.degree.nameBSen
thesis.degree.levelUndergraduateen
dc.contributor.committeeMemberZhou, Hong-Cai
dc.type.materialtexten
dc.date.updated2018-05-23T15:36:27Z


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