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dc.contributor.advisorSuchodolski, Jan S
dc.contributor.advisorSteiner, Jörg M
dc.creatorGuard, Blake Crosby
dc.date.accessioned2018-02-05T21:11:37Z
dc.date.available2019-08-01T06:52:34Z
dc.date.created2017-08
dc.date.issued2017-06-23
dc.date.submittedAugust 2017
dc.identifier.urihttp://hdl.handle.net/1969.1/165797
dc.description.abstractChronic gastrointestinal disease in dogs can manifest itself in many different ways including vomiting, diarrhea, and weight loss. Bile acid dysmetabolism has recently been recognized as an important component of chronic gastrointestinal disease (e.g., Crohn’s disease, Ulcerative Colitis, Irritable Bowel Syndrome, and Inflammatory Bowel Disease) in humans. The aim of this research was to evaluate bile acid dysmetabolism in chronic enteropathy of dogs. An assay for the measurement of unconjugated fecal bile acids using gas chromatography coupled with mass spectrometry was developed. The assay was accurate and reproducible. The percent of unconjugated secondary bile acids were significantly decreased in dogs with chronic enteropathy (p=0.0161), with approximately 60% of dogs having bile acid dysmetabolism. The percent of unconjugated secondary bile acids significantly increased in patients with chronic enteropathy after steroid therapy (p=0.0183). The effect of cholestyramine, a bile acid sequestrant, was evaluated for the ability to alter the fecal bile acid pool in healthy dogs. The concentration of secondary bile acids significantly increased in feces of healthy dogs administered cholestyramine (p=0.0183). These results demonstrate that a subset of dogs with chronic enteropathy show fecal bile acid dysmetabolism, and further studies are warranted to evaluate the use of bile acid sequestrants in clinical cases.en
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.subjectbile acidsen
dc.subjectchronic enteropathyen
dc.subjectinflammatory bowel diseaseen
dc.subjectmicrobiomeen
dc.subjectprimary bile acidsen
dc.subjectsecondary bile acidsen
dc.subjectcholestyramineen
dc.subjectbile acid sequestrantsen
dc.subjectGC/MSen
dc.subjectuntargeted metabolomicsen
dc.titleMicrobial Characterization, Metabolomic Profiling, and Bile Acid Metabolism in Healthy Dogs and Dogs with Chronic Enteropathyen
dc.typeThesisen
thesis.degree.departmentVeterinary Small Animal Clinical Sciencesen
thesis.degree.disciplineBiomedical Sciencesen
thesis.degree.grantorTexas A & M Universityen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.levelDoctoralen
dc.contributor.committeeMemberJergens, Albert E
dc.contributor.committeeMemberWebb, Craib B
dc.contributor.committeeMemberJayaraman, Arul
dc.type.materialtexten
dc.date.updated2018-02-05T21:11:37Z
local.embargo.terms2019-08-01
local.etdauthor.orcid0000-0002-3545-7778


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