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dc.contributor.advisorGomes, Carmen
dc.creatorSummerlin III, Harvey Newell
dc.date.accessioned2018-02-05T21:10:43Z
dc.date.available2019-08-01T06:53:38Z
dc.date.created2017-08
dc.date.issued2017-06-27
dc.date.submittedAugust 2017
dc.identifier.urihttp://hdl.handle.net/1969.1/165775
dc.description.abstractA growing population and demand for food force agricultural leaders to look for alternative water sources. Wastewater reuse could be introduced into commercial operations, if regulations and measures are in place to ensure food safety. The objective of this project was to grow and irrigate lettuce with wastewater to track the fate of Escherichia coli and AP205 bacteriophage during and after cultivation to assess their fate and transport. Subsequently, quantitative microbial risk assessment (QMRA) was performed to estimate risk of illness to the public. Contamination levels in foliage, leachate, and soil were directly (P < 0.05) related to initial concentrations of microorganisms in the irrigation water. E. coli concentrations during post-harvest storage (14 days at 4 oC) of foliage increased by over 400%, while AP205 concentrations decreased more than 2 logs. From randomly selected E. coli colonies, in all four biomass types, 81% and 34% showed resistance to ampicillin and cephalothin, respectively. QMRA revealed significant health risks associated with lettuce consumption. E. coli concentrations were used as a fecal indicator bacteria to estimate levels of 6 common pathogens in wastewater and AP205 concentrations were used to estimate norovirus and rotavirus levels. Norovirus and Giardia largely contributed to the 0.8 probability of illness developing from infection, while norovirus and rotavirus showed a 0.24-0.43 probability of illness developing from infection, when using E. coli and AP205 concentrations, respectively. Results show that non-traditional water usage for fresh produce cultivation can pose risks to humans, if standards are not in place to control pathogen contamination levels.en
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.subjectWastewateren
dc.subjectProduce Irrigationen
dc.subjectPathogen Prevalenceen
dc.subjectAntibiotic Resistance Bacteriaen
dc.subjectMicrobial Risk Assessmenten
dc.subjectBacteriophageen
dc.titleFoodborne and Antibiotic Resistant Pathogens Prevalence During Fresh Produce Production Using Non-Traditional Water Sources: Fate, Transport, and Risk Assessment Studyen
dc.typeThesisen
thesis.degree.departmentBiological and Agricultural Engineeringen
thesis.degree.disciplineAgricultural Systems Managementen
thesis.degree.grantorTexas A & M Universityen
thesis.degree.nameMaster of Scienceen
thesis.degree.levelMastersen
dc.contributor.committeeMemberLacey, Ronald
dc.contributor.committeeMemberCastell-Perez, Elena
dc.contributor.committeeMemberKarthikeyan, Raghupathy
dc.type.materialtexten
dc.date.updated2018-02-05T21:10:44Z
local.embargo.terms2019-08-01
local.etdauthor.orcid0000-0001-6959-3754


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