| dc.description.abstract | Adipose tissue plays a major role in energy storage and Adipokine secretion to maintain systemic balance. However, adipocytes that experience hypertrophy or hyperplasia have negative effects on the processes of obesity-associated metabolic syndromes. In addition, adipose tissue macrophages (ATMs) have been reported to regulate adjacent adipocytes via various cell-cell communications including, microRNAs, which have been discovered to exist in extracellular space. In my research, I studied the effects polarized macrophages have on adipogenesis. By obtaining quantitative real time-PCR (qRT-PCR) results, I monitored the growth of adipocytes through measuring their levels of adipogenesis genes such as Adiponectin, and FAS, chronologically. Moreover, oil red O staining assay was applied to record lipid formation in adipocytes as evidence of adipocyte maturation. By comparing adipocytes response to macrophage-conditioned medium (CM) it was shown that macrophages suppress adipogenesis. The various types of macrophages had varying degrees of suppression on adipogenesis, which leads to the need for further investigation of the mechanism of how this happens. | en |
