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dc.contributor.advisorSu, Chin B
dc.creatorMcBride, Daniel
dc.date.accessioned2017-08-21T14:36:08Z
dc.date.available2019-05-01T06:07:21Z
dc.date.created2017-05
dc.date.issued2017-04-10
dc.date.submittedMay 2017
dc.identifier.urihttp://hdl.handle.net/1969.1/161382
dc.description.abstractA versatile STED microscope using a supercontinuum light source for selecting various excitation and depletion wavelengths needed for different fluorescent dyes was designed and built. Using a 0.9 NA microscope objective and 100 nm diameter beads, 90 nm de-convoluted lateral super-resolution was achieved. A major drawback of traditional STED microscopy is the high depletion power required, which alters sample integrity and causes dye bleaching. A new version that should dramatically decrease the required depletion power is proposed and built. This version uses a common vortex phase plate for both the excitation and depletion beams which also improves mechanical stability of the overlapping focal spots. Rigorous electromagnetic and rate equation analysis shows that the required depletion optical power is decreased by a factor of about six in comparison with traditional method for achieving the same lateral super-resolution. In a separate project, in order to introduce more optical power into a NSOM probe without damaging the probe, epoxy heat sinks were fabricated on the probe tip. Optical imaging and optical power measurements were used to verify that the NSOM probe was not damaged when 405 nm light, at an input power of 21.4 mW, was coupled into the NSOM probe. The NSOM probe was used to ex-pose though 93 nm thick photoresist. The maximum scan rate was approximately 300 μm/sec with a 100 nm aperture NSOM probe.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.subjectSTED
dc.subjectNSOM
dc.titleA Method to Dramatically Decrease the Required Depletion Power of STED Super-Resolution Microscopy and High Power Operation of NSOM Probes
dc.typeThesis
thesis.degree.departmentElectrical and Computer Engineering
thesis.degree.disciplineElectrical Engineering
thesis.degree.grantorTexas A & M University
thesis.degree.nameDoctor of Philosophy
thesis.degree.levelDoctoral
dc.contributor.committeeMemberKameoka, Jun
dc.contributor.committeeMemberYeh, Alvin
dc.contributor.committeeMemberHan, Arum
dc.type.materialtext
dc.date.updated2017-08-21T14:36:08Z
local.embargo.terms2019-05-01
local.etdauthor.orcid0000-0002-5290-4437


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