SNP Discovery and Mapping QTLS Associated with Root Traits and Morphological Traits in Tomato
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Tomato (Solanum lycopersicum L.) is an economically important vegetable crop worldwide and like many other crops, its productivity is severely impacted by abiotic stresses. Establishment of an efficient root system is a means to minimize these negative impacts under stress environments, especially in a low nutrient and drought-prone environment. However, there is little information about genetic control of root traits in tomato. In our study, a segregating F2 population derived from a cross between an advanced breeding line RvT1 (Solanum lycopersicum) and a wild species Solanum cheesmaniae was used to map root traits and other morphological traits, which allowed the study of the genetic basis for several root traits in tomato. We applied Genotyping-By-Sequencing (GBS) methodology to discover single nucleotide polymorphisms (SNPs) for constructing a linkage map, which was composed of 742 SNPs and covered 1319.47 cM with an average distance of 1.78 cM between adjacent markers. We have identified 27 QTLs for the root and shoot traits. One common QTL (flanking by chr04_3261417) associated with root length, root surface area, root volume, root fresh weight and root dry weight was identified, which could be a useful marker to screen these traits simultaneously. Our results suggested root traits were regulated by several major QTLs and a suite of small-effect QTLs.
Xie, Limeng (2016). SNP Discovery and Mapping QTLS Associated with Root Traits and Morphological Traits in Tomato. Master's thesis, Texas A & M University. Available electronically from