Evaluation of Cell Culture Exercise Protocols using a Mechanical Stretch System for Analysis of DEPTOR Expression
MetadataShow full item record
The generation and maintenance of muscle mass is a subject of interest across many scientific fields, including cancer, diabetes, and exercise physiology. Protein synthesis is a key step in the development and maintenance of muscle tissue, and alterations that affect this process can induce a wide array of significant and often deleterious results. A wide variety of regulatory proteins are involved in muscle tissue formation and protein synthesis. One such regulatory protein of particular interest is DEPTOR, which acts as a negative inhibitor of mTOR expression and decreases the rate of protein synthesis. Recent data in our lab has revealed that DEPTOR is differentially expressed in scenarios of diabetes, disuse, and exercise, suggesting that DEPTOR may have a significant physiological role as a key regulator of protein synthesis. In this review, an analysis of numerous exercise protocols for achieving various physiological outcomes is provided, specifically targeting protocols whose results may provide an ideal basis for evaluating changes in DEPTOR expression in the provided scenarios. Following this evaluation, an analysis of different inhibitory compounds and substrates will be provided to give a basis for additional means of altering DEPTOR expression, which can be incorporated into exercise protocols to study the combined effects of exercise and molecular interactions on DEPTOR signaling. The information provided in this review is intended to provide an understanding of various aspects of mechanically simulated exercise that can be used as parameters to study DEPTOR expression in muscle in vitro.
Wudeck, Elyse Victoria (2013). Evaluation of Cell Culture Exercise Protocols using a Mechanical Stretch System for Analysis of DEPTOR Expression. Honors and Undergraduate Research. Available electronically from