MicroRNA Regulation of IGF-1

dc.creator	McEnery, Kayla Anne Filan
dc.date.accessioned	2013-06-04T16:14:15Z
dc.date.available	2013-06-04T16:14:15Z
dc.date.created	2013-05
dc.date.issued	2013-02-04
dc.date.submitted	May 2013
dc.identifier.uri	https://hdl.handle.net/1969.1/148875
dc.description.abstract	Stroke is the fourth leading cause of death worldwide. Low levels of IGF-1, a known neuroprotectant, correspond to aging and increased severity of stroke. We have previously demonstrated that there is an inverse relationship between the presence of anti-let7f or anti-miR-1 and levels of IGF-1. Further, the localization of let-7f was found to be within the microglial cell population. We hypothesize that let7f and miR-1 directly bind to the 3’ UTR region of IGF-1. Microglial cells (EOC-20) will be transfected with an IGF-1 clone with a luciferase tag. IGF-1 synthesis following treatment with antagomirs to either miR-1 or let7f will be tested by a luciferase assay.	en
dc.format.mimetype	application/pdf
dc.subject	microRNA, MiRNA, IGF-1, stroke	en
dc.title	MicroRNA Regulation of IGF-1	en
dc.type	Thesis	en
thesis.degree.department	Biochemistry/Biophysics	en
thesis.degree.discipline	Biochemistry	en
thesis.degree.grantor	Honors and Undergraduate Research	en
dc.contributor.committeeMember	Sohrabji , Farida
dc.type.material	text	en
dc.date.updated	2013-06-04T16:14:15Z