Browsing by Subject "RNAi"
Now showing items 1-12 of 12
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A Study of Nicotiana Benthamiana Protein Interactions with Tomato Bushy Stunt Virus
(2013-04-29)Two Tomato bushy stunt virus (TBSV) proteins, P19 and P22, have been found to interact with the Nicotiana benthamiana host proteins Hin19 and HFi22 in yeast two,hybrid assays. To determine functional roles of these interacting ... -
Anti-viral RNAi and its suppression in plants.
(2010-01-16)As a defense against viral infection, plants are thought to use RNA-induced silencing complexes (RISCs) to target and cleave viral RNA. To counteract this, some viruses have evolved proteins to inhibit RISC-mediated activity, ... -
Cloning, Immunolocalization and Functional Analyses of Calcitonin Receptor 1 (AedaeGPRCAL1; Diuretic Hormone 31 Receptor) in Females of Mosquito Aedes aegypti (Diptera: Culicidae)
(2013-04-26)G protein-coupled receptors (GPCRs) are composed of seven transmembrane domains and play an essential role in regulating physiological functions and mediating responses to environmental stimuli, biogenic amines, ... -
Comparison of gene expression in pre-implantation bovine embryos either injected or transfected with a siRNA targeted against E-cadherin
(2009-05-15)The ability to create transgenic livestock is a tremendous benefit in scientific research for many disciplines including functional genomics, pharmaceutical synthesis and development of enhanced production animals. Transgenes ... -
Functional Analysis of an Integrated GTPase Regulating the Cellular Pool and Distribution Profile of Intraflagellar Transport Particles in Chlamydomonas Reinhardtii
(2012-12-11)Cilia and flagella are sensory organelles, found in the majority of eukaryotic organisms that play a vital role in the general physiology, health and early development of humans. Intraflagellar transport (IFT) is tasked ... -
Gene silencing in cancer cells using siRNA : genetic and functional studies
(Texas A&M University, 2004-09-30)Sequence-specific small interfering RNA (siRNA) duplexes can be used for gene silencing in mammalian cells and as mechanistic probes for determining gene function. Transfection of siRNA for specificity protein 1 (Sp1) in ... -
Identification and Characterization of Adult Aedes aegypti Midgut Peritrophic Matrix Heme-Binding Proteins
(2018-12-04)Bloodfeeding is a high risk, high reward process that has evolved in many arthropods. The adult female Aedes aegypti mosquito- a hematophagous arthropod, is the principle vector of dengue, chikungunya, yellow fever and ... -
Identification and Characterization of Bovine Pol III Promoters to Express a Short-Hairpin RNA
(2010-12-08)The use of molecular biology as a means to advance agriculture has proven beneficial in many fields. However, the development lentiviral vectors that utilize a livestock promoter to express short hairpin RNA (shRNA) has ... -
Identification of ARGONAUTES Involved in Antiviral RNA Silencing in Nicotiana benthamiana
(2012-12-11)ARGONAUTE proteins (AGOs) are generally accepted as key components of the post transcriptional gene silencing mechanism, also involved in plant antiviral defense. Except for reports on the antiviral roles of AGO1, AGO2 and ... -
Immunolocalization and in vivo Functional Analysis by RNAi of the Aedes Kinin Receptor in Female Mosquitoes of Aedes aegypti (L.) (Diptera, Culicidae)
(2012-02-14)The evolution of the blood feeding adaptation has required precise coordination of multiple physiological processes in the insect, such as reproduction, behavior, digestion and diuresis. These processes are under careful ... -
Isolation and Characterization of an Anti-viral RISC in Plants
(2006-07-11)As a defense against viral infection, plants are only very recently thought to use an RNA-induced silencing complex (RISC) as part of the RNA interference (RNAi) pathway to target and cleave viral RNA. To counteract this, ... -
PROPERTIES OF THE TOMBUSVIRUS MOVEMENT PROTEIN AND RNAi SUPPRESSOR THAT INFLUENCE PATHOGENESIS
(2010-01-16)Tomato bushy stunt virus (TBSV) provides a good model system to investigate molecular virus-host interactions in plants. P22 and P19 proteins encoded by TBSV contribute to multiple invasion-associated functions. Green ...