Characterization Of The Local Electrical Environment In An Electrically-guided Protein Patterning System Incorporating Antifouling Self-assembled Monolayer
Abstract
In earlier research in our lab, the manipulation of microtubules on gold patterned silicon wafers was achieved by E-beam lithography, Poly (ethylene glycol) self assembled monolayers (PEG-SAMs) and electrophoresis. To develop a technique for delicate single microtubule manipulation, further studies need to be done on PEG-SAMs and electrophoresis. As a foundation of this goal, we examined the electric field in an aqueous solution between two planar electrodes and the compatibility of the antifouling property of PEG-SAMs with the electric field. For this purpose, the distribution of microbeads was analyzed using a Boltzmann distribution. The amount of adsorbed microtubules on a PEG-SAM was examined to test the compatibility of the antifouling property of a PEG-SAM with concomitant exposure to electric field. It is shown that the product of the electric field and the effective charge of the microbead does not have a linear relation with the applied electric potential but an exponentially increasing function with respect to the potential. The antifouling property of the PEG-SAM was not retained after an exposure to the electric field.
Subject
Protein patterningElectrophoresis
Electrostatic screening
Counterion
Self assembled monolayer
Antifouling
Citation
Park, Jinseon (2010). Characterization Of The Local Electrical Environment In An Electrically-guided Protein Patterning System Incorporating Antifouling Self-assembled Monolayer. Master's thesis, Texas A&M University. Available electronically from https : / /hdl .handle .net /1969 .1 /ETD -TAMU -2010 -08 -8520.