Show simple item record

dc.creatorHan, Junyanen_US
dc.date.accessioned2012-02-14T22:17:45Zen_US
dc.date.accessioned2012-02-16T16:14:50Z
dc.date.available2012-02-14T22:17:45Zen_US
dc.date.available2012-02-16T16:14:50Z
dc.date.created2009-08en_US
dc.date.issued2012-02-14en_US
dc.date.submittedAugust 2009en_US
dc.identifier.urihttp://hdl.handle.net/1969.1/ETD-TAMU-2009-08-7016en_US
dc.description.abstractA xanthene-BODIPY cassette is used as a ratiometric intracellular pH reporter for imaging protein-dye conjugates in living cells. A model was hypothesized to explain the pH-dependent energy transfer efficiencies from the donor to the acceptor based on the electronic chemistry data. Sulfonation conditions were developed for BODIPY dyes to give water-soluble functionalized monosulfonation and disulfonation donors. A water-soluble TBET cassette, which has good photophysical properties, was synthesized using a bissulfonated BODIPY dye as the donor, and their applications for in vitro protein labeling is achieved. Chemoselective cross-coupling reactions were demonstrated for C-S bonds in the BODIPY dye, and similar reactions were applied to make the acceptor of the watersoluble cassette. Chemiluminescent energy transfer cassettes based on fluorescein and Nile Red were synthesized and their spectral properties were studied. Pep-1 (also known as Chariot), R8 (which is not often used as a non-covalent protein carrier), and a new synthesized compound, Azo-R8, was used for the study of non-covalent delivery of four different proteins into mammalian cells. Data from confocal spectroscopy revealed that all three carriers are effective for translocating protein cargos into live cells. At 37 dgrees C, import into endocytic compartments dominates, but at 4 degrees C weak, diffuse fluorescence is observed in the cytosol indicative of a favorable mode of action.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.subjectenergy transferen_US
dc.subjectcell penetrating peptideen_US
dc.subjectpH probesen_US
dc.titleDesign, Syntheses and Biological Applications of Through-bond Energy Transfer Cassettes and Novel Non-covalently Cell Penetrating Peptidesen_US
dc.typeThesisen
thesis.degree.departmentChemistryen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.grantorTexas A&M Universityen_US
thesis.degree.nameDoctor of Philosophyen_US
thesis.degree.levelDoctoralen_US
dc.contributor.committeeMemberBurgess, Kevinen_US
dc.contributor.committeeMemberPellois, Jean-Philippeen_US
dc.contributor.committeeMemberRomo, Danielen_US
dc.contributor.committeeMemberSingleton, Danielen_US
dc.type.genrethesisen_US
dc.type.materialtexten_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record