[Effects of the Myc Oncogene on Cell Growth]
Abstract
The effects of expression of the Ha-ras oncogene on the growth of murine 3T3-NR6 fibroblasts were examined. Doubling times and rates of protein synthesis and degradation were measured in six cell lines. Three cell lies were Ha-ras transformed (as determined by Southern and Western Blotting), two were parent lines (3T3 – Swiss, Webster and 3T3-NR6), and the other was found to carry the Ha-ras oncogene but was not expressing it.
The transformed cell lines, NR6-NR*6.6-A, NRG-NR*6.6-B, and NR6-NR*6.6-C, were all found to have lower serum requirements than the nontransformed cells. Also, the transformed cells exhibited higher rates of protein synthesis than the non-transformed cells (in 1% and 10% serum), while the rates of protein degradation were virtually identical for all cell lines. In addition, the effect of platelet-derived growth factor (PDGF) and insulin on protein synthesis and degradation were determined. It was found that PDGF at a concentration of 1ng/ml induced a slight increase in overall protein accumulation (synthesis – degradation), but the increase was the same for all cell lines. The effects of insulin varied, both at 10⁻⁹ M and 10⁻⁷ M, with the cell line NR6-NR*6.6-C showing the only substantial increase in protein accumulation.
Description
[Program year: 1986-1987]Digitized from print original stored in HDR
Thesis is handwritten
Subject
Ha-ras oncogeneprotein synthesis
protein degradation
serum requirements
insulin
platelet-derived growth factor
Citation
James, Guy (1987). [Effects of the Myc Oncogene on Cell Growth]. [Undergraduate Fellows]. Available electronically from https : / /hdl .handle .net /1969 .1 /CAPSTONE -JamesG _1987.