Isolation and Characterization of a Phosphomannose Isomerase Mutant in Yeast
Abstract
A mutant of Saccharomyces cerevisiae lacking 92 percent of its phosphomannose isomerase (E.C.5.3.1.8.) activity was isolated after mutation with E.M.S.. The mutant was found to have the same auxotrophic requirements as the parent strain A364a. Thermostability studies indicated the mutation was in the structural genome and in vivo incorporation studies with C¹⁴ (C-1)mannose indicated a similar rate of decarboxylation as the parent strain.
Description
Program year: 1977-1978Digitized from print original stored in HDR
Citation
Adams, Sjoerd (1978). Isolation and Characterization of a Phosphomannose Isomerase Mutant in Yeast. University Undergraduate Fellows. Available electronically from https : / /hdl .handle .net /1969 .1 /CAPSTONE -AdamsS _1978.