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dc.contributor.advisorMurphy, Keith E.en_US
dc.creatorBoggs, Rene' Michelleen_US
dc.date.accessioned2008-10-10T20:58:34Z
dc.date.available2008-10-10T20:58:34Z
dc.date.created2008-05en_US
dc.date.issued2008-10-10
dc.identifier.urihttp://hdl.handle.net/1969.1/85979
dc.description.abstractMicroRNAs (miRNAs) play a vital role in differentiation, proliferation and tumorigenesis by binding to messenger RNAs (mRNA) and inhibiting translation. To initiate an investigation into the identification of miRNAs in the domestic dog, an emerging model for human disease, a comparison of the human and canine genetic databases was conducted. The bioinformatics work revealed significant conservation of miRNA genes between the two species. Proof of principle experiments, including serial dilutions and sequencing, were performed to verify that primers made to amplify human mature miRNAs can be used to amplify canine miRNAs, providing that the mature sequences are conserved. TaqMan® Real-time RT-PCR, a sensitive and specific method, was used to isolate the first miRNA mature products from canine tissues. The expression levels of miR-17-3p, miR-17-5p, miR-18, miR-19a, miR-19b, miR-20, and miR-92 were evaluated in five canine tissues (heart, lung, brain, kidney, and liver). Because miRNAs have been found to act as both tumor suppressors and oncogenes in several different cancers, expression patterns of ten miRNAs (miR-15a, miR-16, miR-17-5p, miR-21, miR-29b, miR-125b, miR-145, miR-155, miR-181b, let-7f) known to be associated with human breast cancer were compared between malignant canine mammary tumors (n=6) and normal canine mammary tissue (n=10). Resulting data revealed miR-29b and miR-21 to have a statistically significant (p<0.05) up-regulation in cancerous samples. Overall expression patterns showed nine of the ten miRNAs follow the same pattern of expression in the domestic dog as the human, while the miR-145 expression does not show a difference between the normal and cancerous samples.en_US
dc.format.mediumelectronicen_US
dc.language.isoen_USen_US
dc.publisherTexas A&M Universityen_US
dc.subjectbreast canceren_US
dc.subjectRT-PCRen_US
dc.subjectmiRNAen_US
dc.subjectcanineen_US
dc.subjectgeneticsen_US
dc.titleMicroRNA expression in canine mammary canceren_US
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentVeterinary Pathobiologyen_US
thesis.degree.disciplineVeterinary Microbiologyen_US
thesis.degree.grantorTexas A&M Universityen_US
thesis.degree.nameDoctor of Philosophyen_US
thesis.degree.levelDoctoralen_US
dc.contributor.committeeMemberLong, Charles R.en_US
dc.contributor.committeeMemberWomack, Jamesen_US
dc.contributor.committeeMemberPorter, Weston W.en_US
dc.contributor.committeeMemberKier, Annen_US
dc.type.genreElectronic Dissertationen_US
dc.type.materialtexten_US
dc.format.digitalOriginborn digitalen_US


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