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dc.contributor.advisorMcKnight, Thomasen_US
dc.creatorThomas, Beth Elene Armstrongen_US
dc.date.accessioned2007-04-25T20:17:02Z
dc.date.available2007-04-25T20:17:02Z
dc.date.created2006-12en_US
dc.date.issued2007-04-25
dc.identifier.urihttp://hdl.handle.net/1969.1/5029
dc.description.abstractPhosphate is an essential but limited macronutrient for all plants. In response to limited levels of phosphate, plants have developed highly specialized developmental, biochemical, and molecular responses. To further expand our knowledge of the phosphate starvation induced signal transduction pathway in plants, the expression of the phosphate starvation inducible Purple Acid Phosphatase 1 (PAP1) gene was studied in transgenic Arabidopsis. While few components have been identified regulating gene expression under phosphate starvation conditions in plants, one cis regulatory element recognized by the MYB transcriptions factor Phosphate Starvation Response 1 (PHR1) has been identified in many phosphate starvation induced (PSI) genes. PAP1 and many other genes examined during the course of the mutant characterization contain this cis element. Using the GUS reporter gene under control of the PAP1 promoter, a mutant screen was devised for plants showing abnormal PAP1 response to phosphate nutrition. Three mutant lines were identified and subsequently characterized for the phosphate starvation-induced signal-transduction pathway in Arabidopsis. Two mutants, BT1 and BT2, both with dominant mutations, showed increased GUS staining. The mutations in BT1 and BT2 are tightly linked to the transgene and to each other, but complementation analysis suggested that they are in different genes. Characterization of these mutants indicated that the PSI genes PAP1 and At4 (in BT1 roots), and RNS1 (in BT2 leaves) have alternative or additional methods of regulation other than PHR, even though these genes all contain PHR1 binding sites. A third mutant, BT3, had a phenotype similar to the PAP1 null-mutant and did not show PAP1 phosphatase activity under normal soil-grown conditions. Characterization of BT3 indicates that PAP1, RNS1, and AtIPS1 are not exclusively regulated by PHR1. In an attempt to map the BT3 mutant in a Columbia background by crossing with Landsberg erecta (Ler), it was discovered that the Ler ecotype does not show PAP1 phosphatase activity under normal soil-grown conditions. The PAP1 phosphatase regulatory trait, named BT5, was mapped to a 15,562 bp-region area containing only two genes between the GPA1 and ER markers on Chromosome 2.en_US
dc.format.extent2081267 bytes
dc.format.mediumelectronicen_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherTexas A&M Universityen_US
dc.subjectPhosphate starvationen_US
dc.subjectArabidopsisen_US
dc.titleRegulation of phosphate starvation response in Arabidopsisen_US
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentBiologyen_US
thesis.degree.disciplineBiologyen_US
thesis.degree.grantorTexas A&M Universityen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelMastersen_US
dc.contributor.committeeMemberGriffing, Lawrenceen_US
dc.contributor.committeeMemberPepper, Alanen_US
dc.contributor.committeeMemberShippen, Dorothyen_US
dc.type.genreElectronic Thesisen_US
dc.type.materialtexten_US
dc.format.digitalOriginborn digitalen_US


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