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dc.contributor.advisorCote, Gerard L.en_US
dc.creatorChowdhury, Mustafa Habiben_US
dc.date.accessioned2007-04-25T20:09:00Z
dc.date.available2007-04-25T20:09:00Z
dc.date.created2005-12en_US
dc.date.issued2007-04-25
dc.identifier.urihttp://hdl.handle.net/1969.1/4816
dc.description.abstractRecent advances in nanotechnology and the biotechnology revolution have created an immense opportunity for the use of noble metal nanoparticles as Surface Enhanced Raman Spectroscopy (SERS) substrates for biological sensing and diagnostics. This is because SERS enhances the intensity of the Raman scattered signal from an analyte by orders of 106 or more. This dissertation deals with the different aspects involved in the application of SERS for biosensing. It discusses initial studies performed using traditional chemically reduced silver colloidal nanoparticles for the SERS detection of a myriad of proteins and nucleic acids. It examines ways to circumvent the inherent aggregation problems associated with colloidal nanoparticles that frequently lead to poor data reproducibility. The different methods examined to create robust SERS substrates include the creation of thermally evaporated silver island films on microscope glass slides, using the technique of Nanosphere Lithography (NSL) to create hexagonally close packed periodic particle arrays of silver nanoparticles on glass substrates as well as the use of optically tunable gold nanoshell films on glass substrates. The three different types of SERS surfaces are characterized using UV-Vis absorption spectroscopy, Electron Microscopy (EM), Atomic Force Microscopy (AFM) as well as SERS using the model Raman active molecule trans-1,2-bis(4-pyridyl)ethylene (BPE). Also discussed is ongoing work in the initial stages of the development of a SERS based biosensor using gold nanoshell films for the direct detection of b-amyloid, the causative agent for Alzheimer's disease. Lastly, the use of gold nanoshells as SERS substrates for the intracellular detection of various biomolecules within mouse fibroblast cells in cell culture is discussed. The dissertation puts into perspective how this study can represent the first steps in the development of a robust gold nanoshell based SERS biosensor that can improve the ability to monitor biological processes in real time, thus providing new avenues for designing systems for the early diagnosis of diseases.en_US
dc.format.extent6476396 bytes
dc.format.mediumelectronicen_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherTexas A&M Universityen_US
dc.subjectSERSen_US
dc.subjectRamanen_US
dc.subjectnanoparticlesen_US
dc.subjectnanoshellsen_US
dc.subjectcolloiden_US
dc.subjectNanosphere Lithographyen_US
dc.subjectbeta amyloiden_US
dc.subjectproteinen_US
dc.subjectDNAen_US
dc.subjectcellen_US
dc.titleThe use of Surface Enhanced Raman Spectroscopy (SERS) for biomedical applicationsen_US
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentBiomedical Engineeringen_US
thesis.degree.disciplineBiomedical Engineeringen_US
thesis.degree.grantorTexas A&M Universityen_US
thesis.degree.nameDoctor of Philosophyen_US
thesis.degree.levelDoctoralen_US
dc.contributor.committeeMemberMeissner, Kenithen_US
dc.contributor.committeeMemberTaylor, Henry F.en_US
dc.contributor.committeeMemberWang, Lihongen_US
dc.type.genreElectronic Dissertationen_US
dc.type.materialtexten_US
dc.format.digitalOriginborn digitalen_US


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