Show simple item record

dc.contributor.advisorBinas, Berten_US
dc.creatorAdhikari, Seanen_US
dc.date.accessioned2006-10-30T23:32:14Z
dc.date.available2006-10-30T23:32:14Z
dc.date.created2006-08en_US
dc.date.issued2006-10-30
dc.identifier.urihttp://hdl.handle.net/1969.1/4391
dc.description.abstractHeart-type fatty acid binding protein (H-FABP) is a major fatty acid binding factor in skeletal muscles. Genetic lack of H-FABP severely impairs the esterification and oxidation of exogenous fatty acids in soleus muscles isolated from chow-fed mice (CHOW-solei) and high fat diet-fed mice (HFD-solei), and prevents the HFD-induced accumulation of muscle triglycerides. Here, we examined the impact of H-FABP deficiency on the relationship between fatty acid utilization and glucose oxidation. Glucose oxidation was measured in isolated soleus muscles in the presence or absence of 1 mM palmitate (simple protocol) or in the absence of fatty acid after preincubation with 1 mM palmitate (complex protocol). With the simple protocol, the mutation slightly reduced glucose oxidation in CHOW-muscles, but markedly increased it in HFDmuscles; unexpectedly, this pattern was not altered by the addition of palmitate, which reduced glucose oxidation in both CHOW- and HFD-solei irrespective of the mutation. In the complex protocol, the mutation first inhibited the synthesis and accumulation of triglycerides and then their mobilization; with this protocol, the mutation increased glucose oxidation in both CHOW- and HFD-solei. We conclude: (i) H-FABP mediates a non-acute inhibition of muscle glucose oxidation by fatty acids, likely by enabling both the accumulation and mobilidoes not mediate the acute inhibitory effect of extracellular fatty acids on muscle glucose oxidation; (iii) H-FABP affects muscle glucose oxidation in opposing ways, with inhibition prevailing at high muscle triglyceride contents.zation of a critical mass of muscle triglycerides; (ii) H-FABPen_US
dc.format.extent277507 bytes
dc.format.mediumelectronicen_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherTexas A&M Universityen_US
dc.subjectFABPen_US
dc.subjectfatty aciden_US
dc.subjecttriglycerideen_US
dc.subjectglucoseen_US
dc.subjectsoleus muscleen_US
dc.titleGlucose oxidation in heart-type fatty acid binding protein null miceen_US
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentCouncil of Deansen_US
thesis.degree.disciplineGeneticsen_US
thesis.degree.grantorTexas A&M Universityen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelMastersen_US
dc.contributor.committeeMemberWagner, Galeen_US
dc.contributor.committeeMemberWasser, Jeremy S.en_US
dc.contributor.committeeMemberWu, Guoyaoen_US
dc.type.genreElectronic Thesisen_US
dc.type.materialtexten_US
dc.format.digitalOriginborn digitalen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record