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    Development of tandem time-of-flight instrumentation for the examination of prompt photodissociation of peptides using 193-nm radiation

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    Date
    2006-04-12
    Author
    Morgan, Joseph William
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    Abstract
    The design and incorporation of a decelerating/accelerating cell into a reflectron time-of-flight mass spectrometer is described for the examination of promptly-formed photodissociation products of peptide ions. The analytical utility of prompt 193-nm photodissociation was investigated for model peptides that resemble tryptic digest products, as well as for two sets of homologous peptides. The first of these sets include bradykinin, several bradykinin fragments, and two bradykinin mutants with substituted amino acids. Fragment ion spectra of [M + H]+, [M + Na]+, and [M + Cu]+ were collected for each of these peptides. The second set of homologous peptides has the sequence XVGVAZG, where variable amino acid X was either arginine, histidine, or lysine, and amino acid Z was either proline, serine, or glycine. Photofragment ion spectra obtained using the new mass spectrometer are compared to results of high energy collision induced dissociation (CID) acquired on a high performance commercial instrument. The advantages and disadvantages of prompt photodissociation relative to CID are discussed, as well as the advantages of photodissociation using the modified instrument geometry versus that of the post-source decay focusing method.
    URI
    http://hdl.handle.net/1969.1/3293
    Subject
    Photodissociation
    Instrumentation
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    • Electronic Theses, Dissertations, and Records of Study (2002– )
    Citation
    Morgan, Joseph William (2005). Development of tandem time-of-flight instrumentation for the examination of prompt photodissociation of peptides using 193-nm radiation. Doctoral dissertation, Texas A&M University. Texas A&M University. Available electronically from http : / /hdl .handle .net /1969 .1 /3293.

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