Intein mediated purification of protein
Abstract
The present invention includes methods, compositions, uses, and kits for purifying a protein of interest (POI) comprising contacting a first fusion protein comprising the POI fused to the C-terminus of an intein C-fragment with a second fusion protein comprising an intein N-fragment and a purification tag to form a complex between the first fusion protein and the second fusion protein, cleaving the POI from the intein C-fragment, wherein the protein is released from the complex; and isolating the POI; the present invention also includes fusion proteins and vector.
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Citation
Chen, Zhilei; Ramirez, Miguel A.; Guan, Dongli (2018). Intein mediated purification of protein. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /177269.