Monoclonal antibody against novel epitopes of foot-and-mouth disease virus protein 3ABC and uses thereof
Abstract
This disclosure pertains to isolated antibodies or antigen binding fragments thereof that specifically bind to the 3ABC non-structural protein of Foot-and-Mouth Disease virus (FMDV), wherein the antibodies or antigen binding fragments thereof recognize the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 or SEQ ID NO: 12. Accordingly, this disclosure also pertains to polypeptides having an amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 or SEQ ID NO: 12. Monoclonal antibody Mab 40C8 is also provided. The current disclosure also pertains to methods of detecting FMDV infection in an animal (including assays differentiating infected animals from vaccinated animals (DIVA)) and kits for performing the detection methods. Competitive ELISA kits comprising the antibody or antigen binding fragment thereof and immunoassay plates coated with the polypeptide comprising the amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and/or SEQ ID NO: 12 are also provided.
Subject
C07K 16/1009G01N 33/56983
C07K 2317/34
C07K 2319/21
C12N 2770/32122
C12N 2770/32134
G01N 2333/09
Collections
Citation
Clavijo, Alfonso; Rieder, Aida Elizabeth; Sayed, Abu; Bounpheng, Mangkey A.; Burrage, Thomas G.; Dancho, Brooke A.; Uddowla Blakeney, Sabena (2018). Monoclonal antibody against novel epitopes of foot-and-mouth disease virus protein 3ABC and uses thereof. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /177263.