Method for producing a recombinant baculovirus expression vector
Abstract
A method for producng a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell, is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant transfer vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant transfer vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. The resultant recombinant baculovirus is then used to infect susceptible insects or cultured insect cells and the protein product from the incorporated selected gene is produced from the infection.
Subject
435/320.1536/23.1
536/24.1
C07K 14/005
C07K 14/52
C12N 15/86
C07K 2319/00
C07K 2319/02
C07K 2319/09
C07K 2319/61
C07K 2319/735
C07K 2319/75
C12N 2710/14122
C12N 2710/14143
Collections
Citation
Smith, Gale E.; Summers, Max D. (1989). Method for producing a recombinant baculovirus expression vector. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /176964.