Regulation and evolution of the patatin multigene family : suggestions of combinatorial interactions directing tissue-specific gene expression

Simple item page
dc.contributor.advisorPark, William D.
dc.contributor.committeeMemberCobb, D. Gregory
dc.contributor.committeeMemberDavis, Scott K.
dc.contributor.committeeMemberFunkhouser, Edward A.
dc.creatorMay, Gregory DeWitt
dc.date.accessioned2024-02-09T21:19:12Z
dc.date.available2024-02-09T21:19:12Z
dc.date.issued1992
dc.descriptionVitaen
dc.descriptionMajor subject: Plant Physiology and Plant Biotechnologyen
dc.description.abstractWhile much has been written about the evolution of new tissues or organs, little is known about the origin of the regulatory elements that direct gene expression in new structures. Questions also arise about how new regulatory elements are integrated into the complex physiology of new tissues. To examine these questions, the patatin multigene family was used as a model system. Patatin is a family of lipid acyl hydrolases that accounts for 30-40% of the total protein in potato tubers. The Class-I genes, which produce the majority of the patatin protein in tubers, can also be induced to express at high levels in stems and leaves by 0.2 -0.3 M sucrose. However, under normal conditions, they are almost totally tuber-specific. To examine how "tuber-specific" genes could have evolved from regulatory elements in non-tuberizing species, the expression of the endogenous patatin genes in tomato and tobacco were compared to that of potato Class-I patatin promoters using transgenic plants. Patatin promoters were also isolated and characterized from tobacco and evolutionarily divergent species of wild potato. These promoters contain sequences characteristic of both the Class-I and Class-II patatin genes. However, their patterns of expression are unlike those of any previously reported patatin genes. They were neither sucrose inducible nor tuber-specific and were wound-inducible, unlike potato Class-I patatin genes which are turned off by wounding. Because the major soybean vegetative storage protein (VSP) serves similar roles in soybeans to that served by patatin in potatoes, vsp promoter expression in transgenic potato plants was also examined. Although vsp is normally expressed in soybean leaves, it was tuber-specific when introduced into potato plants. Like Class-I patatin, vsp could be induced to express in leaves by sucrose, but unlike patatin, vsp could also be induced by methyl jasmonate, systemin and oligourinides...en
dc.format.digitalOriginreformatted digitalen
dc.format.extentxiii, 135 leavesen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.identifier.oclc31521990
dc.identifier.urihttps://hdl.handle.net/1969.1/DISSERTATIONS-1450743
dc.language.isoeng
dc.publisher.digitalTexas A&M University. Libraries
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectMajor plant physiology and plant biotechnologyen
dc.subject.classification1992 Dissertation M466
dc.subject.lcshGenetic regulationen
dc.subject.lcshPotatoesen
dc.subject.lcshGerm-plasm resourcesen
dc.subject.lcshPlant proteinsen
dc.subject.lcshPotatoesen
dc.subject.lcshDevelopmenten
dc.subject.lcshGenetic aspectsen
dc.titleRegulation and evolution of the patatin multigene family : suggestions of combinatorial interactions directing tissue-specific gene expressionen
dc.typeThesisen
dc.type.genredissertationsen
dc.type.materialtexten
thesis.degree.disciplinePlant Physiology and Plant Biotechnologyen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.levelDoctorialen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.namePh. Den

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
1450743.pdf
Size:
19.26 MB
Format:
Adobe Portable Document Format
Download

Collections

Digitized Theses and Dissertations (1922–2004)