A homeotic gene expression map in the central nervous system of larval Drosophila melanogaster: a quest for the holy engrailed
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Date
2013-02-22
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Texas A&M University
Abstract
The expression of homeotic genes during early animal development determines the identity of specific populations of cells and thus the regions of the developing body. Furthermore, their expression is regulated so that it occurs at specific developmental time points. Previous studies demonstrated that the expression of homeotic genes could be used as molecular markers for identifying individual sets of neuroblasts within the embryonic central nervous system of Drosophila melanogaster. In this study cellular maps of the expression of the homeotic gene engrailed within the thoracic ventral ganglion of Drosophila larvae were created. Additionally, a staining technique used to label both engrailed expressing cells and dividing neuroblasts within the thoracic region was optimized. This double staining technique will allow for the identification of individual populations of dividing neuroblasts with the engrailed expressing cells acting as landmarks within the brain.
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Includes bibliographical references (leaves 36-37).
Includes bibliographical references (leaves 36-37).
Keywords
biochemistry and genetics., Major biochemistry and genetics.