The in vitro cultivation of Babesia bigemina utilizing bovine cells in culture
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1976
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Abstract
Babesia bigemina in vitro cultivation experiments utilizing primary and continuous monolayer cultures were conducted. Experiments to infect normal non-infected cells by in vitro inoculation using fresh or stabilate B bigemina-infected blood as inoculum were conducted with primary monolayer cultures of bovine spleen, lymph node, hemal node, and fetal kidney and continuous monolayer cultures of African Green Monkey kidney cells Vero. When fresh infected blood was used as inoculum the B bigemina organisms dissociated from their host erythrocytes by day 2 and extracellular parasites were indentifiable for up to 5 days on the surface of the cultured cells. When stabilate preparations were used as inoculums the majority of the parasites remained intraerythrocytic with few extracellular parasites being observed. Babesia bigemina-infected erythrocytes present in the inoculum were observed for up to 14 days on the surface of the cultured cells; however, the parasites were degenerative and pyknotic in appearance. No differences were observed between the various types of cultured cells and there was no evidence that parasitic infection of culture cells or multiplication of organisms took place in the original cultures or subsequent subcultures. Experiments with primary monolayer cultures derived from B bigemina-infected calves were conducted with spleen, lymph node, hemal node and leucocyte cultures. Five days after culture seeding B bigemina organisms could be found only in splenic monolayer cultures and could be identified in such cultures for 11 days post culture..
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Veterinary Microbiology