Activity Of Transfer RNA Methyltransferases During Insect Metamorphosis

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dc.contributor.advisorTsutsui, Ethel Ashworth
dc.creatorAlexander, Victor Charles
dc.date.accessioned2022-04-01T16:00:08Z
dc.date.available2022-04-01T16:00:08Z
dc.date.issued1977
dc.descriptionProgram year: 1976/1977en
dc.descriptionDigitized from print original stored in HDRen
dc.description.abstractTransfer RNA (tRNA) methyltransferases were partially purified from Manduca sexta prepupa larvae and 2-day-old pupae. The purification procedure involved pH 5 precipitation, gel filtration to remove endogenous inhibitors and activators, and ammonium sulfate precipitation. To ensure maximal activation of all preparations, 0.36 M ammonium acetate was used in the reaction mixture. Escherichia coli B tRNA was the methyl accepting substrate, and ¹⁴CH₃-S-adenosylmethionine was the methyl donor. The prepupa larvae had a specific activity of 140 units/mg protein. The 2-day-old pupae had a specific activity of 430 units/mg protein.en
dc.format.extent17 pagesen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/1969.1/CAPSTONE-SykoraE_1998
dc.subjectTransfer RNAen
dc.subjectManduca sextaen
dc.subjectprepupa larvaeen
dc.subjectpupaeen
dc.titleActivity Of Transfer RNA Methyltransferases During Insect Metamorphosisen
dc.title.alternativeACTIVITY OF TRANSFER RNA METHYLTRANSFERASES DURING INSECT METAMORPHOSISen
dc.typeThesisen
dc.type.materialtexten
thesis.degree.departmentBiochemistryen
thesis.degree.grantorUniversity Undergraduate Fellowen
thesis.degree.levelUndergraduateen

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