Construction of the Diploid, Tetraploid and Integrated Diploid-tetraploid Genetic Linkage Maps in Roses Using Simple Sequence Repeat (SSR) Markers
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Date
2014-01-03
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Abstract
This study uses polymorphic microsatellites (SSR) to elucidate the similarities among the diploid and tetraploid rose genomes by comparing their maps and clarifying the predominant inheritance patterns (disomic versus tetrasomic) seen in the tetraploid population.
One hundred and eight out of 175 SSRs were polymorphic in both the OBxWOB26 (Old Blush x (‘Basye’s Thornless’ x ‘Old Blush’) diploid backcross population and the GGFC (‘Golden Gate’ x ‘Fragrant Cloud’) tetraploid full-sib population. Of these 69 fluorescently labeled SSRs and 5 morphological traits were used which generated 107 loci and 5 trait loci with 99 diploid population progeny. The tetraploid map was constructed with SSRs and AFLPs with 131 tetraploid progeny using the single dose restriction fragment (SDRF) analysis. The degree of preferential chromosome pairing in the tetraploid population was examined by looking at the segregation ratios among the double-dose markers (DDMs) as well as the ratio of loci in repulsion vs coupling phase using single-dose markers (SDMs). These approaches showed that there was a combination of disomic and tetrasomic inheritance.
A diploid, a tetraploid and an integrated diploid-tetraploid genetic linkage map were developed from two populations using JoinMap 4 with the cross pollination option. In the diploid map, 7 integrated linkage groups covered a length of 352.3 cM with an average chromosome size of 50.3 cM. The morphological traits, prickles on stem (prickles), recurrent bloom (RB)) and flower type (Blfo) were mapped on the Chr LG3 which matched with the ICM (Integrated consensus map) published by Spillers et al., (2010). Moreover, 5 out of the 69 SSR markers (RhJ404, H9_B01, RW11E5, RW8B8 and RhE3) were mapped to two or more loci each on different chromosomes of the diploid map. In the tetraploid map, 174 out of 346 (50%) loci of single-dose markers (SDMs) and double-dose markers (DDMs) were mapped on a length of 883.4 cM with 9 linkage groups. Sixty anchor SSR markers were used to join the diploid and tetraploid maps which included 215 loci with a map length of 632 cM. Synteny of common SSRs and morphological traits, prickles, RB, Blfo, powdery mildew resistance (PM) and petal number (PN) on the integrated diploid-tetraploid map with the ICM, the GGFC and the K5 map demonstrated the collinear alignment among these maps.
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Rosa, genetic linkage map, diploid, tetraploid, SSR, single-dose marker (SDM)