The Identification of Five Seedlings Hyper-responsive to Light (SHL), and Characterization of SHL7
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Date
2010-01-16
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Abstract
Light is one of the major environmental factors that controls plant development, through
a process known as photomorphogenesis. Plants perceive light via photoreceptors, and
the information used to direct a myriad of developmental responses. Analysis of mutants
defective in photomorphogenic responses elucidates the complex interactions between
light and plants. Previous genetic screens have yielded a class of mutants which exhibit
exaggerated responses to ambient light, designated shl (seedling hyper-responsive to
light). The following work encompasses the identification of five new shl mutants, a
detailed examination of one of these mutants (shl7), and of the SHL7 gene. The mutants
were isolated in a low-white light screen of seedlings derived from T-DNA mutagenesis.
Each of the mutants exhibits a heritable hyper-responsive phenotype in low-white light,
but displays minimal effects in darkness. For each, a putative site of T-DNA insertion has
been located. In addition to a low-white light phenotype, the shl7 mutant exhibits a mild
hyper-responsive phenotype to 670 nm red and 735 nm far-red light, but significant
hyper-responses to 420 nm blue light. SHL7 encodes a small, unique, and previously
undescribed protein annotated as At4g04925. GFP protein fusion analysis indicates that
the protein is localized to mitochondria.
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photomorphogenesis
cryptochrome